Coding

Part:BBa_M50438

Designed by: Gabriella Schreiner   Group: Stanford BIOE44 - S11   (2018-04-25)


beta pinene synthase sequence

The beta pinene synthase sequence codes for the enzyme which allows bacteria to convert GPP to beta pinene. This will enable us to modify E. coli to produce beta pinene, which has a pine scent and repels mosquitoes. We will test the presence of the enzyme with a western blot and test for beta pinene repellence efficacy using mosquitos. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 616
    Illegal EcoRI site found at 790
    Illegal EcoRI site found at 1068
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 616
    Illegal EcoRI site found at 790
    Illegal EcoRI site found at 1068
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 616
    Illegal EcoRI site found at 790
    Illegal EcoRI site found at 1068
    Illegal BamHI site found at 1606
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 616
    Illegal EcoRI site found at 790
    Illegal EcoRI site found at 1068
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 616
    Illegal EcoRI site found at 790
    Illegal EcoRI site found at 1068
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1488


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